Cross reactivity antibody1/12/2024 1-3 Programmed death-1 receptor (PD-1), an immune inhibitory receptor expressed on activated B cells, T cells and myeloid cells, 4 has critical roles in regulating stimulatory and inhibitory signals in the immune system. In silico modeling were conducted to explain the different binding modes of the 2 antibodies, suggesting a potential mechanism of the antibody cross-species binding.īlockade of a specific pathway that shields tumor cells from immune response is promising in the treatment of multiple cancers, including advanced melanoma, ovarian cancer and advanced non-small cell lung cancer. The hPD-1 epitopes of the 2 functional antibodies were also compared with the binding region on hPD-1 that is responsible for PD-L1 interaction. The epitope identification was based on PD-1-binding ELISA and supported by affinity ranking of surface plasmon resonance results. To quickly identify the epitopes of the 2 antibodies, we used alanine scanning and mammalian cell expression cassette. To facilitate studies in murine models, we developed a functional antibody against both human and murine PD-1, and compared the epitopes of such antibody to a counterpart that only bound to hPD-1. The anti-PD-1 antibodies currently approved for clinical use or under development bind to human PD-1 (hPD-1), but not murine PD-1. Of the inhibitory checkpoints in the immune system, programmed death 1 (PD-1) is one of the most promising targets for cancer immunotherapy.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |